Syphilis is a sexually transmitted (venereal) disease caused by the spirochete Treponema Pallidum. The disease can also be transmitted congenitally thereby attaining its importance in antenatal screening. The test contains Syphilis antigen-coated particles and syphilis Antigen coated on the membrane.
Serological tests for nontreponemal antibodies such as VDRL, RPR, TRUST, etc. are useful as screening tests. Test for Treponema-specific antibodies such as TPHA, FTA-ABS, and rapid Treponema antibody tests are gaining importance as screening as well as confirmatory tests because they detect the presence of antibodies specific to Treponema Pallidum.
The one-step Syphilis card test utilizes the principle of immunochromatography, a unique two-site immunoassay on a membrane. The test contains a membrane strip, which is precoated with recombinant Treponema Pallidum antigens (17KDa, 15KDa) on the test band region.
The patient sample and sample diluent move further on the membrane leading to the formation of a burgundy-colored band at the test region which confirms a positive test result. The absence of this colored band in the test region indicates a negative test result. The unreacted conjugate and the unbound complex if any along with rabbit IgG gold conjugate move further on the membrane and are subsequently immobilized by the goat antirabbit antibodies coated at the control region of the membrane assembly, forming a burgundy-colored band. The control band serves to validate the test results.
Test Device, Desiccant, Sample Dropper, Assay Diluent, and Product Insert
STORAGE AND STABILITY
The kit can be stored at 2-40°C till the expiration date printed on the sealed pouch. DO NOT FREEZE.